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two-color microarray-based gene expression analysis manual version 5.5  (Agilent technologies)


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    Agilent technologies two-color microarray-based gene expression analysis manual version 5.5
    Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from <t>microarray</t> analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.
    Two Color Microarray Based Gene Expression Analysis Manual Version 5.5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two-color microarray-based gene expression analysis manual version 5.5/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    two-color microarray-based gene expression analysis manual version 5.5 - by Bioz Stars, 2026-05
    90/100 stars

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    1) Product Images from "RNA isolation method for single embryo transcriptome analysis in zebrafish"

    Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish

    Journal: BMC Research Notes

    doi: 10.1186/1756-0500-3-73

    Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.
    Figure Legend Snippet: Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.

    Techniques Used: Isolation, Microarray

    Dissecting zebrafish development with microarray analysis . (a) Eight selected embryos ranging from the 16-cell to 8-somite stage. (b) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bars, foreground signal and scatter board bars, background signal). (c) Principal component analysis (PCA) on unnormalized log 2 ratio data (test/reference) showing a 'developmental' curve starting at the 16-cell stage and ending at the 8-somite stage. (d) Spearman correlations between the samples reflect the developmental distance.
    Figure Legend Snippet: Dissecting zebrafish development with microarray analysis . (a) Eight selected embryos ranging from the 16-cell to 8-somite stage. (b) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bars, foreground signal and scatter board bars, background signal). (c) Principal component analysis (PCA) on unnormalized log 2 ratio data (test/reference) showing a 'developmental' curve starting at the 16-cell stage and ending at the 8-somite stage. (d) Spearman correlations between the samples reflect the developmental distance.

    Techniques Used: Microarray



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    two-color microarray-based gene expression analysis manual version 5.5  (Agilent technologies)
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    Agilent technologies two-color microarray-based gene expression analysis manual version 5.5
    Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from <t>microarray</t> analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.
    Two Color Microarray Based Gene Expression Analysis Manual Version 5.5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two-color microarray-based gene expression analysis manual version 5.5/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    two-color microarray-based gene expression analysis manual version 5.5 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.

    Journal: BMC Research Notes

    Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish

    doi: 10.1186/1756-0500-3-73

    Figure Lengend Snippet: Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.

    Article Snippet: Each hybridization mixture was made up from 300 ng 'Test' and 300 ng 'Reference' sample according to the Two-Color Microarray-Based Gene Expression Analysis Manual version 5.5 (Agilent Technologies).

    Techniques: Isolation, Microarray

    Dissecting zebrafish development with microarray analysis . (a) Eight selected embryos ranging from the 16-cell to 8-somite stage. (b) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bars, foreground signal and scatter board bars, background signal). (c) Principal component analysis (PCA) on unnormalized log 2 ratio data (test/reference) showing a 'developmental' curve starting at the 16-cell stage and ending at the 8-somite stage. (d) Spearman correlations between the samples reflect the developmental distance.

    Journal: BMC Research Notes

    Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish

    doi: 10.1186/1756-0500-3-73

    Figure Lengend Snippet: Dissecting zebrafish development with microarray analysis . (a) Eight selected embryos ranging from the 16-cell to 8-somite stage. (b) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bars, foreground signal and scatter board bars, background signal). (c) Principal component analysis (PCA) on unnormalized log 2 ratio data (test/reference) showing a 'developmental' curve starting at the 16-cell stage and ending at the 8-somite stage. (d) Spearman correlations between the samples reflect the developmental distance.

    Article Snippet: Each hybridization mixture was made up from 300 ng 'Test' and 300 ng 'Reference' sample according to the Two-Color Microarray-Based Gene Expression Analysis Manual version 5.5 (Agilent Technologies).

    Techniques: Microarray